ฐานข้อมูลอันตรกิริยาระหว่างสมุนไพรกับยาแผนปัจจุบัน

Part Used : ไม่ระบุ

Activity : P-GLYCOPROTEIN INHIBITION

Solvent/Active Compound : Reserpine

Type of experiment : -

Type of animal : -

Type of study : -

N(Total) : -

N(Treatment) : -

Sex : -

Age : -

Route : -

Dose/Conc.(herb) : 15 micromolar

Duration : -

Type of interaction : Pharmacokinetics

Interaction with drug : -

Dose/Conc.(drug) : -

Result : Positive

Remark : Results: Reserpine increased the uptake of doxorubicin in P-glycoprotein overexpressing cells, indicating that reserpine inhibited the efflux function of P-glycoprotein and using molecular docking, found that reserpine bound with even higher binding energy to P-glycoprotein and EGFR than the control drugs verapamil (P-glycoprotein inhibitor) and erlotinib (EGFR inhibitor).

Note : CCRF and P-glycoprotein-overexpressing CEM/ADR5000 cells were incubated with 10 micromolar doxorubicin with and without reserpine (15 micromolar) for 3 hours. Flow cytometry has been used to measure the retention of doxorubicin. Doxorubicin is substrate of P-glycoprotein and its inherent fluorescence was used to assess the efflux activity of this drug transporter.

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