Part Used : ผล
Activity : CYP3A4 INHIBITION
Solvent/Active Compound : methanol
Type of experiment : in vitro
Type of animal : -
N(Total) : -
N(Treatment) : -
Route : -
Dose/Conc.(herb) : 1.65 mg/ml of water extract powder
Duration : -
Type of interaction : Pharmacokinetics
Interaction with drug : Erythromycin*/Erythrocin
Dose/Conc.(drug) : -
Remark : Among the MeOH-soluble fractions, Piper nigrum leaf showed the highest inhibitory activity against CYP3A4 (91.7%) and Punica granatum against CYP2D6 (98.1%). The water extract MeOH-soluble fraction showed inhibitory activity more than 70% were fractionated with EtOAc. The most potent inhibitory activity against CYP3A4 (IC50 avlue of 25 mg/ml) was found for the extract of Pi. nigrum leaf, while that of Catharanthus roseus showed the most potent inhibitory effect against CYP2D6 (IC50 value of 11 mg/ml).
Note : Ketoconazole and quinidine (1 micromolar) were used as the positive control for CYP3A4 and CYP2D6, respectively.